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Research
8.
Confirmation of Chlamydophila abortus in
infected cell culture using Indirect
Immunofluorescence technique -
Sreeja R Nair, M Mini, Nair G Krishnan, V
Jayaprakasan
Vet World. 2011; 4(10): 473-474
doi: 10.5455/vetworld.2011.473-474
Abstract
Chlamydophila abortus (C. abortus) is an important
abortifacient agent in bovines and ovines.
Clinical diagnosis of the disease is often
difficult. An early diagnosis can be achieved
based on direct demonstration of the organism in
clinical material and through the cultural
recovery of the organism in embryonated chicken
egg. For confirmatory diagnosis antigen detection
methods or serological techniques can be adopted.
The present study is aimed at the confirmatory
diagnosis of C. abortus infection by indirect
immunofluorescence technique following the
isolation of the organism in cell culture.
Specific apple green fluorescing inclusions of C.
abortus in McCoy cell lines was detected from 72 h
to 96 h post infection employing anti-chlamydial
group specific monoclonal antibodies. Thus, a
confirmatory diagnosis of the infection was
possible with this study.
Keywords:
Chlamydophila abortus, Indirect immunofluorescence,
McCoy cell lines, Monoclonal antibodies.
